Article Figures & Data
Figures
- Figure 1
Exogenous did not disturb its endogenous expression after C-type natriuretic peptide (CNP) treatment in mesangial cells. (A) ELISA was used to detect the supernatant level of CNP; (B) real-time PCR was used to detect the mRNA level of CNP; (C) the protein expression of CNP was measured by western blot. *P<0.05, significantly different from the corresponding control group (0 pM CNP).
P<0.05, significantly different from the corresponding low-dose group (10 pM CNP). - Figure 2
C-type natriuretic peptide (CNP) inhibited mesangial cells (MCs) proliferation and collagen-IV (Col-IV) expression. (A) 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays were used to measure the growth of MCs; (B) ELISA was used to detect the supernatant level of Col-IV; (C) Real-time PCR was used to detect the mRNA level of CNP; (D) the protein expression of CNP was measured by western blot. *P<0.05, significantly different from the corresponding control group (0 pM CNP).
P<0.05, significantly different from the corresponding low-dose group (10 pM CNP). - Figure 3
C-type natriuretic peptide (CNP) induced the elevation of the expressions of CNP receptor signaling (natriuretic peptide receptor (NPR)-B, cyclic guanosine monophosphate-dependent protein kinase (CGK) II and NPR-C) in mesangial cells (MCs). (A, D, G) ELISA was used to detect the supernatant levels of NPR-B, CGK II and NPR-C; (B, E, H) Real-time PCR was used to detect the mRNA levels of NPR-B, CGK II and NPR-C; (C, F, I) the protein expressions of NPR-B, CGK II and NPR-C were measured by western blot. *P<0.05, significantly different from the corresponding control group (0 pM CNP).
P<0.05, significantly different from the corresponding low-dose group (10 pM CNP). - Figure 4
C-type natriuretic peptide (CNP) downregulated the neutral endopeptidase (NEP) expression in mesangial cells. (A) ELISA was used to detect the supernatant level of NEP; (B) real-time PCR was used to detect the NEP mRNA; (C) the protein expression of NEP was measured by western blot. *P<0.05, significantly different from the corresponding control group (0 pM CNP).
P<0.05, significantly different from the corresponding low-dose group (10 pM CNP).
Tables
- Table 1
List and sequence of primers
Variable Sequence Product length (bp) C-type natriuretic peptide Sense 5′-AACAGAGGCTTCTCCCGAAAA-3′ 149 Antisense 5′-GTCTTGCACTCTAGCAGCGT-3′ Natriuretic peptide receptor-B Sense 5′-TGACCCCGACCTGCTGTTA-3′ 150 Antisense 5′-CGAACCAGGGTACGATAATGG-3′ Natriuretic peptide receptor-C Sense 5′-AGACTACGCCTTCTTCAACATTG-3′ 90 Antisense 5′-GCTTCAAAGTCGTGTTTGTCTCC-3′ Cyclic guanosine monophosphate-dependent protein kinase II Sense 5′-GAACCTCACCACTGATGCTCT-3′ 104 Antisense 5′-GCTCCTTCAAATGGTACTCCCG-3′ Neutral endopeptidase Sense 5′-AGAAGAAACAGCGATGGACTCC-3′ 93 Antisense 5′-CATAGAGTGCGATCATTGTCACA-3′ Collagen-IV Sense 5′-GGACTACCTGGAACAAAAGGG-3′ 240 Antisense 5′-GCCAAGTATCTCACCTGGATCA-3′ Glyceraldehyde-3-phosphate dehydrogenase Sense 5′-GGAGCGAGATCCCTCCAAAAT-3′ 197 Antisense 5′-GGCTGTTGTCATACTTCTCATGG-3′
