Figure 5 Pemetrexed and cisplatin in sequence generates T cell inflamed tumor microenvironment. qRT-PCR analysis of IFNB1(A), CXCL10 (B), TNF-α (C), and IL-12 (D) gene expression. (E) Immunohistochemical staining of the tumor sections for infiltrated CD8+ T cells. (F) PD-L1 expression in tumors was analyzed by western blot. Data are presented as mean±SD, n=6 for each group. &P<0.05, &&P<0.01, &&&P<0.001. *P<0.05, **P<0.01, ***P<0.001. IFNB1, interferon beta 1; CXCL10, C-X-C motif chemokine ligand 10; IL-12, interleukin 12; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; qRT, quantitative reverse transcription; mRNA, messenger RNA; PD-L1, programmed death molecular ligand-1.