Midwest Clinical and Translational Research Meeting of CSCTR and MWAFMR

Deployed PFO closure device during procedure

Bacterial phylum abundance in the intestinal microbiome

Intestinal barrier dysfunction in CPB versus controls

Short chain fatty acid levels in CPB versus controls

Intestinal eicosanoids levels in CPB versus controls

Systemic cytokine levels in CPB versus controls

Mechanisms of thrombosis in patients with lipoedema and lymphedema. Platelets are hyperactivated through PAR1 agonist (TRAP6) and thromboxane receptor agonist (U46619) in both lipedema and lymphedema patients. Platelets are hyperactivated with P2Y12 receptor agonist (ADP) and GPVI receptor agonist (CRP) only in lymphedema patients

Mechanisms of thrombosis in patients with lipoedema and lymphedema. Fibrin generation in platelet-deplete plasma from subjects

Demographic distribution of study population

Forest plot demonstrating the risk of mortality for patients on various combinations of medications (Risk Ratio *p<0.05, **p<0.01)

Plot showing association of increasing age with worse outcomes (p<0.01 for all outcomes)

ECG’s from second (a), third (b), and fourth (c) admissions depicting the presence and resolution of diffuse ST-elevations in anterior and inferior leads, with PR depressions in leads II, III, aVF, V5, and V6

Chest X-Ray showing left-sided pleural effusion (a). CT angiogram of chest with contrast depicting pericardial effusion (b, c). Cardiac MRI demonstrating a clear demarcated circumferential rim of delayed enhancement in the pericardium (d)

Te preferred reporting items for systematic review and meta-analysis (PRISMA) flow chart for the included studies

Forest plot of BCG and total atopic diseases. Random effects model forest plot shows ORs and 95% Cis for the association between BCG vaccination and total atopic disease

Forest plot of the association between BCG vaccination and asthma. Random effects model forest plot shows ORs and 95% Cis for the association between BCG vaccination and asthma. OR = 0.73, 95% CI 0.58 to 0.92. M-H = Mantel-Haenszel; OR = odds ratio; CI = confidence interval

Forest plot of the association between BCG vaccination and rhinitis. Random effects model forest plot shows ORs and 95% CIs of the association between BCG vaccination and rhinitis. OR = 0.99, 95% CI 0.81 to 1.21. M-H = Mantel-Haenszel; OR = odds ratio; CI= confidence interval

Forest plot of the association between BCG vaccination and eczema. Random effects model forest plot shows ORs and 95% Cis for the association between BCG vaccination and eczema. OR = 1.09, 95% CI 0.60 to 1.96. M-H = Mantel-Haenszel; OR = odds ratio; CI = confidence interval

Left upper extremity nodular skin lesion

Nodular skin lesion evolving to hemorrhagic bulla

Nodular hemorrhagic bulla evolving into cribriform appearing ulcer

Isoleucine restriction robustly reduces body weight in both male and female geriatric mice starting at 20 months of age. Weekly body weight records of both male (left) and female (right) aged mice starting their respective dietary intervention at 20 months of age. A robust decrease followed by stabilization within a month is seem in both isoleucine restriction groups. A decrease in body weight was also observed in the protein restriction group, but the effect size is relatively less

Isoleucine restriction induces leanness in aged mice of both sexes. Small animal MRI is used to determine the% body composition of mice before dietary intervention and every 3 weeks. It was observed that both male and female mice fed an isoleucine restriction diet become much leaner than their control counterparts. The effect of a protein restriction diet appears to be lessened in female mice

Isoleucine restriction improves glucose tolerance in both male and female aged mice In a glucose tolerance assay, intraperitoneal injection of glucose was better tolerated in animals fed either an isoleucine restriction diet or a protein restriction diet. The benefits of a protein restriction diet was more significant in females

Isoleucine restriction induces cardiac remodeling in aged female mice In females only, echocardiogram experiments revealed a decrease in cardiac stroke volume which was compensated by an increase in heart rate

Isoleucine and protein restriction exhibited distinct modulation of enzymatic phosphorylation pathways. A brief evaluation of whole heart protein phosphorylation downstream of mTORC1 found differences between male mice fed an isoleucine restriction diet and a protein restriction diet

AFMR graph

Survival kaplan-meyer curve

Multiplex immunofluorescence showing significant difference in intra-tumoral versus adjacent thyroid tissue expression of PD-1, PD-L1 (p<0.05) amongst 17 patients with differentiated thyroid cancer

Multiplex immunofluorescence showing more FOXP3+ T regulatory cells, CD68+ macrophages, PD-L1 expression in patient with distant metastases amongst 17 patients with differentiated thyroid cancer

Multiplex immunofluorescence of a differentiated thyroid cancer tissue specimen demonstrating PD-1 staining along the leading edge of the tumor

‘A guide to managing your high blood pressure’ brochure

‘Signs and symptoms’ tearsheet

Infectious disease specialists’ peer- review daptomycin stewardship recommendation acceptance rates. Of 183 interventions, 93 (51%) were accepted over the 5 year program implementation

IHC findings in intestinal tissue of CITRO miceA) Immunohistochemistry staining for MAA antigen in the intestinal tissue of mice in the CITRO model showed significant increase compared to controls p<0.03. Represented image of 6 replicatesB) Immunohistochemistry staining for cit antigen in the intestinal tissue of mice in the CITRO model showed significant increase compared to controls p<0.001. Represented image of 6 replicatesC) Immunohistochemistry staining for Calprotectin antigen in the intestinal tissue of mice in the CITRO model showed significant increase compared to controls ***p<0.0001. Represented image of 6 replicatesD) Immunohistochemistry staining for Claudin-2 antigen in the intestinal tissue of mice in the CITRO model showed significant increase compared to controls ***p<0.001. Represented image of 6 replicatesE) Immunohistochemistry staining for CD19 antigen in the intestinal tissue of mice in the CITRO model showed significant increase compared to controls ***p<0.001. Represented image of 6 replicatesF) Immunohistochemistry co-localization between MAA, citrulline and IBD proteins in the intestinal tissue from mice in the CITRO model. R-squared value showing the amount of overlap between MAA, citrulline and IBD proteins. A significant increase in colocalization is seen between MAA and Calprotectin compared to Cit and Calprotectin ***p=0.0001. Co-localization between cit and claudin-2 was also increased compared to MAA and claudin-2 ***p<0.0001

Serum Anti-MAA IgG antibodies are increased in the CITRO model compared to control mice. Anti-MAA IgG against MAA-CIT is significantly increased in the CITRO model compared to controls and to MAA and CIT alone

TEER varies by GUT segment. Purple represents patients with cirrhosis and yellow represents non-cirrhotic control

Mortality

Mortality with subgrouping according to ACS patients’ exclusion. ACS: abdominal compartment syndrome

Mortality with subgrouping according to IAH pressure cut off. IAH: intra-abdominal hypertension

Multiorgan organ dysfunction syndrome

Respiratory, renal and cardiovascular failure

PLD2 intracellular pathway. Graphical representation of the established role PLD2 has intracellularly

PEL model results

MSC model results

Enrollment scheme hamidi

Increase in LCS participation after intervention

Referral to LCS program and outcome

UChicago-Moffitt adult acute lymphoblastic leukemia (ALL) cohort. (A) Demographic characteristics of 353 adult ALL patients. (B) Variant allelic fractions of mutations detected in this cohort. (C) The associations between clinical variables and pathogenic mutations are colored by odds ratio. (D) Kaplan-Meier relapse-free and overall survival curves of ALL patients stratified based on the presence of clonal hematopoiesis (CH) mutations stem cells (HSCs). If ARCH is a precursor lesion to ALL, HSCs harboring these mutations are expected to maintain hematopoiesis and give rise to mature non-malignant cells harboring similar mutations. To gain insights into the clonal architecture of hematopoiesis in ARCH-associated ALL, we performed single-cell DNA and protein sequencing using the Mission Bio, lnc. amplicon-based sequencing platform. The data from 4,258 cells of an ARCH-associated T-ALL patient are shown in Figure 2. Cells were clustered by immunophenotype, and five pathogenic mutations involving TP53, PHF6, EZH2, ETV6 and ASXL1 genes were overlayed into individual cells of 11 clusters. Most of the mature lymphoid cells residing in T-, B-, and NK-cell clusters were wild-type, whereas T-ALL blasts and myeloid cells of monocytic, megakaryocytic and granulocytic origins shared these mutations in homozygous and heterozygous states. Histopathologic examination of myeloid compartment did not reveal any signs of myeloid malignancy such as dysplasia

Single-cell DNA+protein sequencing of pre-treatment bone marrow sample from an ARCH-associated T-ALL patient. (A) Uniform manifold approximation and projection (UMAP) plot of 4,258 cells clustered by immunophenotype. Key markers used to distinguish clusters are highlighted on the right. (B) Genotype for five pathogenic mutations overlayed into each cell. HOM, homozygous; HET, heterozygous; WT, wild type

Chest X-ray

CT chest

Peripheral blood smear on the initial presentation showing atypical monocytic cells consistent with acute monocytic leukemia

Warfarin-induced calciphylaxis

Left ankle magnetic resonance imaging

Methenamine silver stain of ankle tissue

CODEX image of glomerulitis. CD8 T cells and CD11c mononuclear phagocytes in glomerulitis in a kidney transplant biopsy

Timeline of inotropic support with intervention

A/B pre-operative X-ray of historical C3-C6 posterior spinal fusion

Post-operative CT scan of CAPDF consisting of posterior spinal fusion revision (A) and C3-C6 ACDF (B)

Pulse sequence diagram

Timing diagram

Cine and tagging pre-scan calibration sensitivity

Control vs. validation

Cine-tag overlay

* Inpatient Day 5 – First administration of doxycycline therapy • ** Inpatient Day 9 – Final administration of doxycycline therapy • Outpatient Day 4- First follow up after hospital discharge • Marked increase in serum creatinine 24 hours after first dose of doxycycline. Serum creatinine begins to downtrend 48 hours after final dose of doxycycline

Marked decrease in GFR 24 hours after doxycycline administration with near threefold increase in BUN. GFR begins to recover 48 hours after final dose of doxycycline. BUN levels begin to decline after GFR exceeds 20 mL/min

Diffuse lymphocytes, monocytes and eosinophils infiltrate between the tubules and are associated with lymphocytic tubulitis. (H&E stain, 20x magnification)

Tamm-Horsfall protein casts are found within tubules and show extravasation into the interstitium associated with an inflammatory infiltrate. (PAS stain, 20x magnification) contributor (figures 3 & 4)- Amy Lynn, MD vice chair of anatomic pathology consultants in laboratory medicine/promedica laboratories

Multivariable model of predictors of 28 day mortality. Ordered forest plot of the predictors, including H2RA group, of mortality in our cohort of sepsis-3 patients

Differential gene expression in human airway epithelial cells

PCR for mRNA levels of calcium binding proteins from stimulated U-937 cells

Western blot from stimulated U-937 cells

PCR for mRNA-fold increase from stimulated U-937 macrophages

ELISA for M1- associated cytokines from stimulated U-937 macrophages

ELISA for M2- associated cytokines from stimulated U-937 macrophages

Ultrasound images of bilateral pre-patellar bursitis

Ultrasound-guided aspiration of bilateral pre-patellar bursaeTransverse view, anterior right knee; needle entry of pre-patellar bursa from 3 o’clock position, prior to aspiration (A) and status post aspiration (B). Hyperechoic margin along synovial lining representing calcium crystal deposition. Transverse view, anterior left knee; needle entry of pre-patellar bursa from 2–3 o’clock position, prior to aspiration (C) and status post aspiration (D)

Right forearm swelling, erythema and skin tightening. Stitch marks are noted after obtaining a biopsy t months after initial encounter with the Rheumatology clinic

Deep section showing the lower portion of the biopsy including the fascia. The section shows inflammation and diffuse thickening of the fascia H&E: 2x

Mixed inflammation, including multiple eosinophils (arrows) in the dermis adjacent to the epidermis H&E: 20x

Deep image depicting lymphocytes, histiocytes, plasma cells, and several eosinophils (encircled) in a focus of inflammation within the thickened fascial tissue. H&E: 40x