TY - JOUR T1 - Midwestern Regional Meeting JF - Journal of Investigative Medicine JO - J Investig Med SP - 784 LP - 886 DO - 10.1136/jim-2018-000745.1 VL - 66 IS - 4 A2 - , Y1 - 2018/04/01 UR - http://hw-f5-jim.highwire.org/content/66/4/784.abstract N2 - B02 Interventions to reduce sedentary behavior at workJacquelyn Kulinski1 Ariel Bodker1 Scott Strath2 Michael Widlansky1 David Gutterman1 1Medical College of Wisconsin, WI 2University of Wisconsin-Milwaukee, WI Objective Sedentary behavior is associated with all-cause and cardiovascular disease (CVD) morbidity and mortality, independent of physical activity. However, the biological mechanisms underlying the deleterious consequences of sedentary behavior are largely unknown. We hypothesized that obese subjects with sedentary desk jobs, when assigned use of a sit-stand desk, will reduce their daily sedentary time and demonstrate improvement in arterial flow-mediated dilation (FMD), an early indicator of CVD. Methods Obese subjects without known CVD were recruited at our institution via electronic flyers and received an adjustable sit-stand desk at work. Activities were quantified objectively with an accelerometer for 7 days at baseline and during the intervention. Subjects were incentivized for accelerometer compliance. FMD of the brachial and femoral arteries and nitroglycerin-mediated dilation of the brachial artery, fasting lipids, HbA1c, CRP, and anthropometrics were measured at baseline and 12 weeks. Paired t-tests were used to compare measurements over time. Results Ten participants were enrolled (90% female, mean age 40±5, mean BMI 34±4). Mean sedentary time at work (Monday-Friday 8 AM-5 PM) decreased over the intervention period by an average of 114±33 minutes per day (p=0.003, figure 1). This correlated with an increase in standing time at work (111±39 to 205±77 minutes per day, p<0.005). Femoral FMD improved an average of 3.6% (4.7±1.8 to 7.1±1.6, p=0.019, figure 2) while there was a non-significant trend toward improvement in brachial FMD (7.7±3.1 to 9.9±1.9, p=0.07). There was no change in (endothelial-independent) nitroglycerin-mediated FMD. A significant reduction in fasting triglyceride level was noted (mean reduction 35±13 mg/dL, p=0.005). There was no significant change in body weight or other anthropometrics, HbA1c, CRP, work-day or 24-hour step counts or moderate and vigorous … ER -