RT Journal Article
SR Electronic
T1 Receptor signaling and neutral endopeptidase are involved in the resistance of C-type natriuretic peptide to human mesangial proliferation and collagen-IV expression
JF Journal of Investigative Medicine
JO J Investig Med
FD BMJ Publishing Group Ltd
SP 1
OP 9
DO 10.1136/jim-2017-000533
VO 66
IS 5
A1 Huang Huang Luo
A1 Cheng Wu
A1 Peng Hu
A1 Yang Fang Wu
A1 Dong Dong Zhang
A1 Si Yan Liu
A1 Guang Mei Jiang
A1 Yao Xu
A1 Yue Wu
A1 Jing Jing Wang
A1 Fei Fei Liu
A1 Wei Wei
A1 Bo Hu
YR 2018
UL http://hw-f5-jim.highwire.org/content/66/5/1.1.abstract
AB C-type natriuretic peptide (CNP) is regarded as a local, paracrine hormone to regulate vascular tone and cell proliferation. Although several in vivo studies have documented that CNP exerts the inhibitory effects on mesangial cells (MCs) proliferation and collagen production, a limited number of studies exist about the resistance of CNP to MCs proliferation in vitro. Besides, whether its receptor signaling and neutral endopeptidase (NEP) are involved remains unclear. In the present study, human MCs were incubated in serum-containing medium in the absence or presence of CNP (0, 10 and 100 pM) for 24, 48 and 72 hours, respectively. CNP administration significantly suppresses MCs proliferation and collagen-IV (Col-IV) expression in a time-dependent and dose-dependent manner. As a down-stream signal molecule of CNP activation, the expressions of natriuretic peptide receptor (NPR)-B, cyclic guanosine monophosphate-dependent protein kinases II and NPR-C were obviously augmented, whereas NEP expression was significantly decreased after CNP treatment. In conclusion, receptor signaling and NEP are involved in the resistance of CNP to human mesangial proliferation and Col-IV expression.