TY - JOUR T1 - Investigation of oral, gastric, and duodenal microbiota in patients with upper gastrointestinal symptoms JF - Journal of Investigative Medicine JO - J Investig Med SP - 870 LP - 877 DO - 10.1136/jim-2020-001642 VL - 69 IS - 4 AU - Jorge Cervantes AU - Majd Michael AU - Bo-Young Hong AU - Aden Springer AU - Hua Guo AU - Burgandy Mendoza AU - Mingtao Zeng AU - Olof Sundin AU - Richard McCallum Y1 - 2021/04/01 UR - http://hw-f5-jim.highwire.org/content/69/4/870.abstract N2 - Disease-associated alterations of the intestinal microbiota composition, known as dysbiosis, have been well described in several functional gastrointestinal (GI) disorders. Several studies have described alterations in the gastric microbiota in functional dyspepsia, but very few have looked at the duodenum.Here, we explored the upper GI tract microbiota of inpatients with upper GI dyspeptic symptoms, and compared them to achalasia controls, as there is no indication for an esophagogastroduodenoscopy in healthy individuals.We found differences in the microbiota composition at the three sites evaluated (ie, saliva, stomach and duodenum). Changes observed in patients with dyspepsia included an increase in Veillonella in saliva, an oral shift in the composition of the gastric microbiota, and to some degree in the duodenum as well, where an important abundance of anaerobes was observed. Metabolic function prediction identified greater anaerobic metabolism in the stomach microbial community of patients with dyspepsia. Proton pump inhibitor use was not associated with any particular genus. Co-abundance analysis revealed Rothia as the main hub in the duodenum, a genus that significantly correlated with the relative abundance of Clostridium, Haemophilus, and Actinobacillus.We conclude that patients with upper GI symptoms consistent with dyspepsia have alterations in the microbiota of saliva, the stomach, and duodenum, which could contribute to symptoms of functional GI disorders.Data are available upon reasonable request. ER -