TY - JOUR T1 - Non-surgical animal model of gastroesophageal reflux disease by overeating induced in mice JF - Journal of Investigative Medicine JO - J Investig Med SP - 1208 LP - 1214 DO - 10.1136/jim-2020-001691 VL - 69 IS - 6 AU - Nu-Ri IM AU - Byoungjae Kim AU - Kwang-Yoon Jung AU - Tae Hoon Kim AU - Seung-Kuk Baek Y1 - 2021/08/01 UR - http://hw-f5-jim.highwire.org/content/69/6/1208.abstract N2 - Previous animal models of gastroesophageal reflux disease (GERD) were not physiological and required a variety of surgical procedures. Therefore, the animal model developed by conditions that are similar to the pathogenesis of GERD is necessary. The aim is to establish a non-surgical animal model with GERD caused by overeating induced in mice. To induce mice to overeat, we designed dietary control protocols including repetitive fasting and feeding. The esophageal tissues were evaluated with GERD markers to prove the establishment of a GERD animal model. Mice fasted every other day (group 2) showed more pronounced overeating feature and demonstrated evident changes similar to the macroscopic and microscopic findings of GERD, the expressions of inducible nitric oxide synthase and substance P were stronger. The higher frequency of fasting and overeating could cause GERD effectively. The dietary control can make mice overeat, which elicits the change of lower esophageal mucosa similar to GERD. Thus, the overeating-induced mouse may be used as a GERD mouse model.All data relevant to the study are included in the article or uploaded as supplementary information. ER -