TY - JOUR T1 - Excessive proliferation and apoptosis of parathyroid cells contribute to primary hyperparathyroidism in rabbit model JF - Journal of Investigative Medicine JO - J Investig Med SP - 1392 LP - 1398 DO - 10.1136/jim-2021-002184 VL - 70 IS - 6 AU - Jing-tao Bi AU - Rong-jie Bai AU - Hui-li Zhan AU - Zhan-hua Qian AU - Li-hua Gong AU - Ya-qi Liu AU - Zhi-xue Zheng AU - Xuan Cai Y1 - 2022/08/01 UR - http://hw-f5-jim.highwire.org/content/70/6/1392.abstract N2 - To explore the molecular pathogenesis of primary hyperparathyroidism (PHPT), we investigated the proliferation and apoptosis of parathyroid cells in a rabbit model of diet-induced PHPT. A total of 120 adult Chinese rabbits were randomly divided into normal diet (Ca:P, 1:0.7) group (control group) or a high-phosphate diet (Ca:P, 1:7) group (experimental group). The thyroid and parathyroid complexes were harvested for 1-month interval from month 1 to month 6. The expression of proliferation markers, including proliferating cell nuclear antigen (PCNA) and cyclin-D1, and B cell lymphoma-2 (Bcl-2), were evaluated by immunohistochemistry in thyroid and parathyroid tissues. Apoptosis was quantified by DNA-fragment terminal labeling. Our results demonstrated that parathyroid cells in the experimental group started proliferating from the end of the 2nd month, the expression of PCNA, Bcl-2, and cyclin-D1 were significantly higher in the PHPT group than those of the control group (p<0.05). Furthermore, the apoptosis index (AI) was positively correlated with the glandular cell count and expression of PCNA in the 6th month in the PHPT group. Overall, our results suggested that excessive proliferation and apoptosis of parathyroid cells may contribute to the pathogenesis of PHPT through PCNA-related, Bcl-2-related, and cyclin-D1-related pathways.No data are available. ER -