TY - JOUR T1 - Southern Regional Meeting, New Orleans, February 18–20, 2016 JF - Journal of Investigative Medicine JO - J Investig Med SP - 488 LP - 634 DO - 10.1136/jim-2015-000035.483 VL - 64 IS - 2 A2 - , Y1 - 2016/02/01 UR - http://hw-f5-jim.highwire.org/content/64/2/488.3.abstract N2 - 481: ASSESSING HEART RATE VARIABILITY AFTER ACUTE ADDERALL ADMINISTRATION IN THE SPRAGUE-DAWLEY RATC McKinnie1K Varner1E Kerut1J McKinnie21Louisiana State University School of Medicine, Metairie, LA2East Jefferson General Hospital, Metairie, LAPurpose of Study The purpose of this study was to determine whether repeated administration of amphetamine would alter function of the autonomic nervous system (ANS) in the rat, and to thereby assess changes in heart rate variability (HRV). We hypothesized that the acute and chronic administration of amphetamine would not only increase heart rate (HR) and systolic blood pressure (BP), but would also decrease HRV which could in time lead to cardiovascular pathology.Methods Used Telemetry blood pressure (BP) transducers were surgically implanted into male Sprague-Dawley rats (n=6). One week later (day 0), each rat received intraperitoneal (ip) injections of saline (0.1 ml) in the morning and afternoon. On days 1–17, amphetamine (2 mg/kg, ip) was administered in both the AM and PM. End-diastolic nadir points were taken from blood pressure waveforms sensed by the telemetry probes in order to generate R-R intervals. From this an in-house computer program was written (MATLAB R2014b) to calculate time domain measures of HR, SDNN, pNN5, and also frequency domain measures of LF, HF, and LF/HF. Average mean arterial pressure and heart rate were computed off-line.Summary of Results BP and HR rose acutely for at least 6 hours after each AM injection throughout the study. Baseline (1 hr before AM injection) BP appears to remain unchanged over time, but baseline HR diminished as the study progressed. The time domain measure pNN5 acutely diminished after each injection, but baseline measures tended to increase over time. LF/HF acutely increased after each AM injection. Over the first 12 days baseline values diminished, but appeared to rise toward the end of the study.Conclusions Acute ip injections of amphetamine increased HR and … ER -