Summary of quantitative real time RT-PCR oligonucleotide primers used in measuring mRNA expression in mitochondrial dynamics, mitochondrial biogenesis and synaptic genes
| Gene | DNA Sequence (5′-3′) | PCR product size |
|---|---|---|
| Mitochondrial dynamics genes | ||
| Drp1 | Forward primer ATGCCAGCAAGTCCACAGAA | 86 |
| Reverse primer TGTTCTCGGGCAGACAGTTT | ||
| Fis1 | Forward primer CAAAGAGGAACAGCGGGACT | 95 |
| Reverse primer ACAGCCCTCGCACATACTTT | ||
| MFN1 | Forward primer GCAGACAGCACATGGAGAGA | 83 |
| Reverse primer GATCCGATTCCGAGCTTCCG | ||
| MFN2 | Forward primer TGCACCGCCATATAGAGGAAG | 78 |
| Reverse primer TCTGCAGTGAACTGGCAATG | ||
| Cyclophilin D | Forward primer AGATGTCAAATTGGCAGGGGG | 91 |
| Reverse primer TGCGCTTTTCGGTATAGTGCT | ||
| Opa1 | Forward primer ACCTTGCCAGTTTAGCTCCC | 82 |
| Reverse primer TTGGGACCTGCAGTGAAGAA | ||
| Mitochondrial biogenesis genes | ||
| PGC1α | Forward primer GCAGTCGCAACATGCTCAAG | 83 |
| Reverse primer GGGAACCCTTGGGGTCATTT | ||
| Nrf1 | Forward primer AGAAACGGAAACGGCCTCAT | 96 |
| Reverse primer CATCCAACGTGGCTCTGAGT | ||
| Nrf2 | Forward primer ATGGAGCAAGTTTGGCAGGA | 96 |
| Reverse primer GCTGGGAACAGCGGTAGTAT | ||
| TFAM | Forward primer TCCACAGAACAGCTACCCAA | 84 |
| Reverse primer CCACAGGGCTGCAATTTTCC | ||
| Reverse primer AGACGGTTGTTGATTAGGCGT | ||
| Synaptic genes | ||
| Synaptophysin | Forward primer CTGCGTTAAAGGGGGCACTA | 81 |
| Reverse primer ACAGCCACGGTGACAAAGAA | ||
| PSD95 | Forward primer CTTCATCCTTGCTGGGGGTC | 90 |
| Reverse primer TTGCGGAGGTCAACACCATT | ||
| Housekeeping genes | ||
| β Actin | Forward primer AGAAGCTGTGCTATGTTGCTCTA | 91 |
| Reverse primer TCAGGCAGCTCATAGCTCTTC | ||
| GAPDH | Forward primer TTCCCGTTCAGCTCTGGG | 59 |
| Reverse primer CCCTGCATCCACTGGTGC | ||