Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of mitochondrial dynamics, mitochondrial biogenesis and synaptic proteins
| Marker | Primary antibody—species and dilution | Purchased from (company, location) | Secondary antibody, dilution, Alexa fluor dye | Purchased from (company, location) |
|---|---|---|---|---|
| Drp1 | Rabbit polyclonal 1:300 | Novus Biological, Littleton, Colorado, USA | Donkey anti-rabbit IgG Alexa Fluor 488 conjugate | Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| Fis1 | Rabbit polyclonal 1:300 | Protein Tech Group, Inc, Chicago, Illinois, USA | Donkey anti-rabbit IgG Alexa Fluor 488 conjugate | Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| Mfn1 | Rabbit polyclonal 1:300 | Protein Tech Group, Inc, Chicago, Illinois, USA | Donkey anti-rabbit IgG Alexa Fluor 488 conjugate | Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| Mfn2 | Rabbit polyclonal 1:200 | Protein Tech Group, Inc, Chicago, Illinois, USA | Donkey anti-rabbit IgG Alexa Fluor 488 conjugate | Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| OPA1 | Rabbit polyclonal 1:500 | Novus Biological, Littleton, Colorado, USA | Donkey anti-rabbit IgG Alexa Fluor 488 conjugate | Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| SYN | Rabbit polyclonal 1:200 | Protein Tech Group, Inc, Chicago, Illinois, USA | Donkey anti-rabbit IgG Alexa Fluor 488 conjugate | Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| PSD95 | Rabbit polyclonal 1:400 | Protein Tech Group, Inc, Chicago, Illinois, USA | Donkey anti-rabbit IgG Alexa Fluor 488 conjugate | Thermo Fisher Scientific, Waltham, Massachusetts, USA |