Table 1

Sequences of primers for TGF-β, S100A4, α-SMA, keratin 7 and GAPDH

SequenceExpected lengths
TGF-βForward: 5’-AACATGATCGTGCGCTCTGCAAGTGCAGC- 3’
Reverse: 5’-AGGACGGACAGACGTGATA-AGGAA- 3’		200 bp
S100A4Forward: 5’-AGGGACAACGAGGTGGACTT- 3’
Reverse: 5’-CTTCCTGGGCTGCTTATCTGG-3’		105 bp
α-SMAForward: 5’-AGCGTGGCTATTCCTTCGTTA-3’
Reverse: 5’-GCCCATCAGGCAACTCGTAA-3’		153 bp
CytokeratinForward: 5’-CTTGGCAGCAGCAGCCTCTA-3’
Reverse: 5’-TCCAGAAACCGCACCTTGTC-3’		239 bp
GAPDHForward: 5’-AGAAGGCTGGGGCTCATTTG-3’
Reverse: 5’-AGGGGCCATCCACAGTCTTC-3’		258 bp

  • α-SMA, smooth muscle aorta; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TGF-β, transforming growth factor-β.