Ranked quality of evidence for each step of AFT.
| Step | Quality of Evidence Ranking |
|---|---|
| Patient Selection Age BMI Gender Menopausal status Cancer treatment and immunosuppressive medications Diabetes | - A A C B C A/B |
| Skin Preparation | C |
| Donor Site Selection | A/B |
| Donor Site Preparation Skin preparation Tumescent fluid recipe Cannula selection Skin entry and closure Interval from injection to harvest | - C B B/C C B |
| Harvest of Fat Graft Skin entry Cannula characteristics Sub-atmospheric pressure setting Modality of energy | - C B/C B B |
| Harvest Ergonomics Force and energy required Rate of retrieval | - C C |
| Harvest Quality Percent viable grafts Ruptured adipocytes SVF quantity Fat quality Fat consistency | - C B B C C |
| Fat Isolation from Blood, Tumescence, Fibrous Particles (gravity, centrifuge, filtration, none) | B |
| Transfer of Fat to Placement Device | B |
| Admixture | B |
| Recipient Site Qualities Skin preparation Local anesthetic injection Skin entry and closure | - C C C |
| Fat Transfer Syringe size Needle or cannula choice and size Depth of placement Speed of placement Volume transferred per recipient potential volume | - C B/C B C B/C |
| Post-procedural Care (compression, elevation, massage, none) | C |
| Perioperative Systemic Pharmacology | B |