Table 2

Identified co-different genus and species associated with RAS which showed a positive difference equal to or higher than 10% (in terms of relative abundance)

Contributing OTU	NSctrl (n=28)	NSras (n=31)	Sras (n=19)	P1 value	P2 value
Genera
Aeromonas	0.0001±0.0001	0.0007±0.0007	0±0	0.025	0.009
Anaerovorax	0.0014±0.0011	0±0	0.0004±0.0004	0	0.021
Candidatus Endomicrobium	0.0032±0.0012	0.0002±0.0002	0.0015±0.0011	0.005	0
Deinococcus	0.0004±0.0004	0.0011±0.0011	0±0	0.03	0.001
Elizabethkingia	0±0	0.0006±0.0004	0±0	0.004	0.016
Ezakiella	0.014±0.0042	0.0007±0.0004	0.0091±0.0053	0.001	0.036
Fretibacterium	0.2809±0.0971	0.0685±0.0154	0.164±0.0369	0.002	0.025
Geobacter	0.0001±0.0001	0.0007±0.0005	0±0	0.025	0.009
Ignavibacteria	0.0035±0.0026	0±0	0.0016±0.0014	0.001	0
Lactococcus	0.0014±0.0006	0.0006±0.0003	0±0	0.037	0.016
Parascardovia	0±0	0.0008±0.0005	0±0	0.001	0.005
Phocaeicola	0.0241±0.0036	0.005±0.001	0.0161±0.0071	0.001	0.048
Rhodospirillales	0.0055±0.0019	0.0001±0.0001	0.0018±0.001	0.002	0
Rickettsiales	0.0001±0.0001	0.0017±0.0015	0.0002±0.0002	0	0
Rikenellaceae	0.1195±0.0232	0.0205±0.0052	0.0675±0.0212	0.001	0.033
Roseburia	0.0759±0.0124	0.0077±0.0021	0.042±0.0151	0.001	0.007
Sediminispirochaeta	0.0058±0.0026	0.0001±0.0001	0.0012±0.0012	0.004	0
Sneathia	0±0	0.0006±0.0006	0.0019±0.0011	0.004	0.007
Species
Acholeplasmatales bacterium_COT_172	0.0544±0.0186	0.0015±0.001	0.0298±0.0144	0.002	0.007
Actinomyces sp_OT_414	0.0027±0.0009	0.0005±0.0002	0.0013±0.0009	0.013	0.032
Aeromonas veronii	0.0001±0.0001	0.0007±0.0007	0±0	0.025	0.009
Alphaproteobacteria bacterium_COT_081	0.0055±0.0019	0.0001±0.0001	0.0018±0.001	0.004	0
Bacteroidales OT_MCE7_164	0.0592±0.0136	0.0131±0.0034	0.0531±0.0186	0.001	0.04
Chlorobi bacterium_COT_046	0.0035±0.0026	0±0	0.0016±0.0014	0.001	0
Deinococcus geothermalis	0±0	0.0011±0.0011	0±0	0.001	0.001
Eubacterium sp_OT_FX028	0.0009±0.0004	0±0	0.0009±0.0009	0	0
Geobacter soli	0.0001±0.0001	0.0007±0.0005	0±0	0.025	0.009
Lachnospiraceae bacterium_FOT_021	0.0759±0.0124	0.0077±0.0021	0.042±0.0151	0.001	0.004
Mycoplasma spermatophilum	0.0001±0.0001	0.0009±0.0008	0±0	0.005	0.002
Peptostreptococcaceae bacterium_COT_221	0.0249±0.0085	0.0046±0.0011	0.0181±0.0068	0.004	0.033
Peptostreptococcaceae bacterium_FOT_028	0.0019±0.0007	0.0001±0.0001	0.0013±0.0009	0	0
Phocaeicola abscessus	0.0241±0.0036	0.005±0.001	0.0161±0.0071	0.001	0.035
Sneathia amnii	0±0	0.0006±0.0006	0.0019±0.0011	0.004	0.007
Spirochaeta sp_COT_314	0.0035±0.0026	0.0001±0.0001	0.0012±0.0012	0.001	0
Synergistetes bacterium_OT_363	0.0544±0.0186	0.0137±0.0031	0.0395±0.0081	0.002	0.006
Tissierella sp_feline_OT_025	0.014±0.0042	0.0007±0.0004	0.0088±0.0053	0.001	0.036
Treponema parvum	0.0029±0.0008	0.0005±0.0003	0.0014±0.0005	0.001	0.019
Treponema sp_5:C:AT040	0.1703±0.0716	0.0254±0.009	0.0605±0.015	0.003	0.044
Treponema sp_9:A:D01	0.0072±0.0016	0.0011±0.0004	0.0036±0.0012	0.002	0.05
Treponema sp_I:C:BA223	0.0115±0.0041	0±0	0.0015±0.0011	0.001	0
Treponema sp_OT_271	0.0544±0.0186	0.0015±0.001	0.0298±0.0144	0.001	0.003

P1 value: NSctrl vs NSras.
P2 value: NSras vs Sras.
NSctrl, non-smoking healthy controls; NSras, non-smoking RAS patients; OTU, operational taxonomic unit; RAS, recurrent aphthous stomatitis; Sras, smoking RAS patients.