Table 2

PCR primers and antibodies used in analyses

Real-time PCR TaqMan primers used in gene expression analyses. Antibodies and isotype control antibodies used to detect cell surface markers by flow cytometry
TaqMan Nkx2–5 primersMm00657783_m1
TaqMan Nppa primers (ANF)Mm01255748_g1
TaqMan Nppb primers (BNP)Mm 01255770_g1
TaqMan TnnC primers (Troponin C)Mm00437111_m1
List of antibodies used for flow cytometry
Flow cytometry antibodyIsotype control
PE anti-mouse CD29PE Armenian Hamster IgG
eFluor660 CD34eFluor660 rat IgG2a, к
PE-Cy7 anti-mouse Ly-6A/E (Sca1)PE-Cy7 rat IgG2a, к
PE anti-mouse CD24PE rat IgG2c, к
PE anti-mouse CD105PE rat IgG2a, к
PE-Cy7 anti-mouse CD117 (c-Kit)PE-Cy7 rat IgG2a, к
PE-Cy7 anti-mouse CD44PE-Cy7 rat IgG2b, к
PE anti-mouse CD90.1 (Thy1.1)PE mouse IgG2a, к
PE anti-mouse CD73PE rat IgG1
PE anti-mouse CD166 (ALCAM)PE rat IgG2a, к
PE-Cy7 anti-mouse CD31PE-Cy7 rat IgG2a, к
PerCP-Cy5.5 anti-mouse CD45PerCP-Cy5.5 rat IgG2b, к
PE-Cy7 anti-mouse CD11bPE-Cy7 rat IgG2b, к
PE anti-mouse CD14PE rat IgG2a, к
PerCp-Cy5.5 anti-mouseTer119(Ly-76)PerCP-Cy5.5 rat IgG2b, к