Elsevier

Genomics

Volume 48, Issue 3, 15 March 1998, Pages 324-329
Genomics

Regular Article
Molecular Cloning, Structural Characterization, and Chromosomal Mapping of the Human LECT2 Gene

https://doi.org/10.1006/geno.1997.5198Get rights and content

Abstract

We originally isolated LECT2 (leukocyte cell-derived chemotaxin 2) as a 16-kDa secreted protein having a human neutrophil chemotactic activity, then cloned human and bovine LECT2 cDNAs and demonstrated the liver-specific expression of the protein. LECT2 is thought to be a multifunctional protein, because it was recently found to be identical to chondromodulin-II, a growth stimulator of chondrocyte cells. We report here the cloning and the structural analysis of the human LECT2 gene. The gene spans approximately 8 kb and consists of four exons and three introns. Primer extension analysis revealed that several transcription initiation sites occur within 70–230 nucleotides upstream of the translation initiation codon. Several transcriptional control sequences relevant to the liver-specific expression have been identified at the 5′ untranslated region of the human LECT2 gene. The human LECT2 gene was mapped to chromosome 5q31.1–q32 by fluorescencein situhybridization. This region contains a cluster of cytokine genes including IL-4, IL-5, and IL-9.

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    Leukocyte cell-derived chemotaxin 2 (LECT2), discovered in 1996, is a chemotactic factor for neutrophils (Yamagoe et al., 1996). Human LECT2 is a 16-kDa basic protein with three intramolecular disulphide bonds; it is expressed in the liver and secreted into the bloodstream (Yamagoe et al., 1998a). LECT2 also plays a role in such disparate processes as the regulation of liver regeneration (Sato et al., 2004a, 2004b), carcinogenesis (Ong et al., 2011; Ovejero et al., 2004; Uchida et al., 1999), hepatic injury (Segawa et al., 2001), and natural killer T (NKT) cell homeostasis (Saito et al., 2004).

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Sequence data from this article have been deposited with the DDBJ/EMBL/GenBank Data Libraries under Accession No. AB007546.

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