Bcl-2 expression by eosinophils in a patient with hypereosinophilia,☆☆,,★★,,♢♢

https://doi.org/10.1016/S0091-6749(98)70344-6Get rights and content

Section snippets

CASE REPORT

A 61-year-old woman had recurrent bilateral periorbital swelling and intermittent urticaria with annular erythematous skin lesions for more than 8 years. The laboratory findings included a marked persistent blood eosinophilia. The absolute eosinophil numbers in blood during the time of these investigations ranged between 2079/mm3 (without glucocorticosteroid therapy) and 600/mm3 (with glucocorticosteroid therapy). Recently, diarrhea, nausea, and vomiting occurred sporadically, and the patient

Antibodies

Anti-Bcl-2 and control IgG1 mAb were obtained from Dako (Zurich, Switzerland); FITC-conjugated anti-Bcl-2 mAb from Ancell Corp (Bayport, Minn); FITC-conjugated control IgG1 mAb from Coulter (Hialeah, Fla); anti-Fas receptor mAb (clone CH-11, IgM) from Immunotech (Marseille, Fance); and anti-CD16 mAb microbeads from Miltenyi Biotec (Bergisch-Gladbach, Germany).

Eosinophil purification and cultures

Eosinophils were purified as previously described.3, 4 Eosinophils were cultured at 1 × 106 /mL for the indicated times with complete

RT-PCR

mRNA expression of Bcl-2 and Fas receptor was studied by using RT-PCR. Primers for Bcl-2 (5 ́-ACA ACA TCG CCC TGT GGA TGA C-3 ́ and 5 ́-ATA GCT GAT TCG ACG TTT TGC C-3 ́) and Fas receptor (5 ́-GTG GGA TCC CAC TTC GGA GGA TTG CTC AAC AAC C-3 ́ and 5 ́-GTG CTG CAG TAT GTT GGC TCT TCA GCG CTA ATA-3 ́) amplifications were synthesized (HSC Biotechnology Service Centre, Toronto, Ontario) according to previously published sequences.3, 4, 5 Primers for β-actin control amplification were obtained from

RESULTS AND DISCUSSION

As demonstrated in Fig 1 (left panel), the purified eosinophils of the presented patient with HES demonstrated, compared with normal control eosinophils, a marked delay of eosinophil death in vitro.

. Spontaneous cell death of HES and normal eosinophils. Left, Delayed spontaneous eosinophil death in a patient with HES. After Fas receptor activation, the HES eosinophils died in a similar manner as normal eosinophils (mean levels of 3 independent experiments are shown) without Fas receptor

Acknowledgements

We thank Martina Weber for excellent technical assistance.

First page preview

First page preview
Click to open first page preview

References (8)

There are more references available in the full text version of this article.

Cited by (0)

From a the Department of Dermatology, Technical University Munich, Munich; and b Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos.

☆☆

*S. G. P. and B. D. contributed equally to this work.

Supported by grants from the Swiss National Science Foundation (32-49210.96); the OPO-Foundation, Zurich; and the Foundation for Scientific Research, University of Zurich.

★★

Reprint requests: Hans-Uwe Simon, MD, Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Obere Strasse 22, CH-7270 Davos, Switzerland.

J Allergy Clin Immunol 1998;102:1037-40.

♢♢

0091-6749/98 $5.00 + 0   1/54/93585

View full text