Abstract
C-type natriuretic peptide (CNP) which potently stimulates particulate guanylate cyclase activity in cultured rat vascular smooth muscle cells (VSMC) inhibited serum-induced DNA synthesis of the cells 10-fold more effectively than alpha-human atrial natriuretic peptide (alpha-hANP). The inhibitory effect of CNP was mimicked by 8-bromo-cGMP. The proliferation of VSMC was also suppressed by CNP more potently than alpha-hANP, while the peptide was less active for cGMP augmentation and for vasorelaxation than alpha-hANP in isolated rat aorta. These results suggest that CNP may be a growth regulating factor of VSMC rather than a vasodilator.
MeSH terms
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Animals
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Aorta, Thoracic / cytology
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Aorta, Thoracic / drug effects
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Aorta, Thoracic / physiology*
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Atrial Natriuretic Factor / pharmacology*
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Cell Division / drug effects
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Cells, Cultured
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Culture Media
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Cyclic GMP / metabolism
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DNA Replication / drug effects
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Growth Inhibitors / pharmacology*
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Humans
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Kinetics
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Male
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Muscle, Smooth, Vascular / cytology
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Muscle, Smooth, Vascular / drug effects
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Muscle, Smooth, Vascular / physiology*
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Natriuretic Peptide, C-Type
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Nerve Tissue Proteins / pharmacology*
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Rats
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Rats, Inbred Strains
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Thymidine / metabolism
Substances
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Culture Media
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Growth Inhibitors
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Nerve Tissue Proteins
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Natriuretic Peptide, C-Type
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Atrial Natriuretic Factor
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Cyclic GMP
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Thymidine