Transformation of dendritic cells from plasmacytoid to myeloid in a leukemic plasmacytoid dendritic cell line (PMDC05)

Norihiro Watanabe; Miwako Narita; Akie Yamahira; Takeshi Nakamura; Nozomi Tochiki; Anri Saitoh; Masami Kaji; Shigeo Hashimoto; Tatsuo Furukawa; Ken Toba; Ichiro Fuse; Yoshifusa Aizawa; Masuhiro Takahashi

doi:10.1016/j.leukres.2010.03.002

Transformation of dendritic cells from plasmacytoid to myeloid in a leukemic plasmacytoid dendritic cell line (PMDC05)

Leuk Res. 2010 Nov;34(11):1517-24. doi: 10.1016/j.leukres.2010.03.002. Epub 2010 Mar 24.

Authors

Norihiro Watanabe¹, Miwako Narita, Akie Yamahira, Takeshi Nakamura, Nozomi Tochiki, Anri Saitoh, Masami Kaji, Shigeo Hashimoto, Tatsuo Furukawa, Ken Toba, Ichiro Fuse, Yoshifusa Aizawa, Masuhiro Takahashi

Affiliation

¹ Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Niigata, Japan.

PMID: 20338639
DOI: 10.1016/j.leukres.2010.03.002

Abstract

We investigated the transformation from pDCs to mDCs in a pDC line (PMDC05) which was established from a patient with pDC leukemia in our laboratory. PMDC05 cells were separated into two fractions according to the expression of BDCA1 and CD123. BDCA1(-)CD123(+) cells were found to be pDC-like cells by their morphology, surface phenotypes, mRNA expression and the function. In addition, BDCA1(-)CD123(+) cells were demonstrated to have a proliferating capacity and revealed the ability to transform to BDCA1(+)CD123(-) cells which showed mDC-like properties. Our data demonstrated the possibility of transformation from pDCs to mDCs in human DC lineage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Lineage
Cell Transformation, Neoplastic*
Cells, Cultured
Dendritic Cells / pathology*
Humans
Leukemia / pathology*